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The Experimental Steps Of Sulfur-Emodin
- Jul 23, 2018 -

Dewaxing Treatment

Remove 10 pieces of paraffin-embedded tissue section to be measured (note: Before paraffin is buried, tissue section must be fixed for 16 hours with 10% Formaldehyde 4 ℃, this step is important)

(select operation) put in 80 ℃ oven, incubate for 30 minutes

(select operation) static 15 minutes at room temperature

 

Follow the table and put it in the small dye jar to incubate.

Dyeing cylinder incubation time xx ml Yiji dewaxing fluid (reagent A) 15 minutes

xx ml Yiji dewaxing fluid (reagent A)

15 minutes.

xx ml Yiji dewaxing fluid (reagent A)

15 minutes.

xx ml Yiji water supplement A (reagent B)

3 minutes.

xx ml Yiji water supplement B (reagent C)

3 minutes.

xx ml Yiji replenishment liquid C (reagent D) 

3 minutes.

Carefully remove the Yiji on the slice C (reagent D)

Careful plus xx micro-liter Yiji cleaning solution (reagent E) on the slice, covered with the whole section of the sample surface

Incubate for 5 minutes at room temperature.

Carefully remove the Yiji solution on the slice (reagent E)